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Related Experiment Video

Updated: May 5, 2026

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
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A U.S. reference for human immunoglobulin E

R Evans

    The Journal of Allergy and Clinical Immunology
    |July 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    A new U.S. reference material for total immunoglobulin E (IgE) has been established using a pooled human serum. This standardized IgE reference material is now available for laboratory use.

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    Related Experiment Videos

    Last Updated: May 5, 2026

    Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
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    Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
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    Area of Science:

    • Allergy and Immunology
    • Biotechnology
    • Clinical Chemistry

    Background:

    • Accurate quantification of total immunoglobulin E (IgE) is crucial for diagnosing allergic diseases.
    • Standardization of IgE measurements requires reliable reference materials.
    • The American Academy of Allergy's Committee on In Vitro Tests initiated efforts to create a U.S. reference standard.

    Purpose of the Study:

    • To establish a U.S. reference material for total IgE.
    • To ensure consistency and accuracy in IgE testing across laboratories.
    • To provide a reliable standard for IgE quantification.

    Main Methods:

    • A large pool of human serum with high IgE titers was collected and screened for hepatitis B surface antigen.
    • The serum pool was subaliquoted, lyophilized, and rigorously tested for total IgE content.
    • The paper radioimmunosorbent test (PRIST) was employed by 14 participating laboratories using identical reagents and the Second International Reference Preparation as a standard.

    Main Results:

    • The characterized IgE reference material demonstrated a mean of means of 899 IU, a median of means of 901 IU, and a weighted mean of 898 IU.
    • The overall coefficient of variation for the total IgE content was 15.3%.
    • The results indicate good inter-laboratory agreement for the established IgE reference material.

    Conclusions:

    • A U.S. reference material for total IgE has been successfully established and characterized.
    • This new IgE standard is available for use by all laboratories to enhance the accuracy of IgE measurements.
    • The availability of this reference material supports improved diagnosis and monitoring of allergic conditions.