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Human immunoglobulin M purification by affinity chromatography on protein A--Sepharose

M A Vidal, F P Conde

Journal of Biochemical and Biophysical Methods

|March 1, 1981

Abstract:

A method is described for preparing IgM from normal serum in two chromatographic steps. A gel filtration column gives a macroglobulin peak containing the IgM, purified by affinity chromatography on protein A--Sepharose. Immunoglobulin recovery is around 50%.

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