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Related Experiment Videos

Antiproteolytic activities found in human tears

J A Anderson, I H Leopold

    Ophthalmology
    |January 1, 1981
    PubMed
    Summary

    Human tears contain an inhibitor that affects papain activity. This tear inhibitor level varied significantly in patients with eye conditions like allergic conjunctivitis and blepharitis.

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    Area of Science:

    • Ophthalmology
    • Biochemistry
    • Immunology

    Background:

    • Human tears possess inherent protective mechanisms.
    • The role of tear components in ocular surface health is crucial.
    • Papain is a thiol-dependent protease relevant to biological systems.

    Purpose of the Study:

    • To investigate the inhibitory activity of human tears against papain.
    • To compare tear papain inhibitory activity in healthy individuals versus those with specific ocular inflammatory conditions.

    Main Methods:

    • Collected human tears from normal subjects and patients with blepharitis, infectious conjunctivitis, allergic conjunctivitis, and herpes simplex.
    • Assayed the papain inhibitory activity in tear samples.
    • Performed statistical analyses (medians, means, geometric means) to compare groups.

    Main Results:

    • Normal tears exhibited papain inhibitory activity.
    • Reduced inhibitory activity was observed in some patients with infectious conjunctivitis and blepharitis.
    • Elevated inhibitory activity was found in patients with herpes simplex and allergic conjunctivitis, with statistically significant differences noted in allergic conjunctivitis and blepharitis.

    Conclusions:

    • Human tear papain inhibitory activity is altered in various external ocular inflammatory diseases.
    • The observed variations suggest a potential functional role for this inhibitor in managing ocular inflammation.

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