Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid massive assembly of tight junction strands

B Kachar, P Pinto da Silva

    Science (New York, N.Y.)
    |July 31, 1981
    PubMed
    Summary

    This study examines how tight junctions form rapidly in rat prostate tissue when incubated at body temperature. The researchers found that new junctions appear within five minutes and are much longer than existing ones. Surprisingly, these junctions form even when protein synthesis is blocked or metabolism is inhibited. The findings suggest that junctions assemble from preexisting components rather than newly made proteins. The authors propose that stress conditions may trigger junction proliferation by mobilizing these components. This work highlights a new mechanism for how epithelial cells adapt to environmental changes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Self-organization of waves and pulse trains by molecular motors in cellular protrusions.

    Scientific reports·2015
    Same author

    Inner ear tissue preservation by rapid freezing: improving fixation by high-pressure freezing and hybrid methods.

    Hearing research·2014
    Same author

    Theory and application of fluorescence microscopy.

    Current protocols in neuroscience·2008
    Same author

    Principles and application of fluorescence microscopy.

    Current protocols in molecular biology·2008
    Same author

    1/f ruffle oscillations in plasma membranes of amphibian epithelial cells under normal and inverted gravitational orientations.

    Physical review. E, Statistical, nonlinear, and soft matter physics·2006
    Same author

    Mechano-electrical transduction: new insights into old ideas.

    The Journal of membrane biology·2006

    Area of Science:

    • Cellular and developmental biology
    • Epithelial tissue dynamics
    • Tight junction assembly mechanisms

    Background:

    Prior research has shown that tight junctions regulate paracellular permeability in epithelial tissues. It was already known that tight junctions form at cell-cell interfaces and are composed of transmembrane proteins. However, the mechanisms driving rapid assembly under stress remain unclear. No prior work had resolved how stress conditions might trigger tight junction proliferation. This gap motivated investigations into whether preexisting components could reorganize rapidly. That uncertainty drove studies on epithelial responses to environmental changes. This paper's contribution lies in demonstrating that new junctions can assemble quickly without new protein synthesis. The findings suggest a previously unknown pool of membrane-associated components may be mobilized.

    Purpose Of The Study:

    The aim of this study is to investigate how tight junctions assemble rapidly in rat prostate tissue under experimental conditions. The specific problem is whether new junctions form from newly synthesized proteins or preexisting components. The motivation is to understand how epithelial cells respond to temperature changes. The study tests if metabolic or protein synthesis inhibitors affect junction assembly. This work addresses whether junction proliferation requires ongoing biosynthesis. The researchers propose to examine assembly rates and junction length changes. They also aim to determine if stress conditions trigger junction proliferation. The results may clarify how epithelial tissues adapt to environmental stress.

    Keywords:
    tight junctionsepithelial cellscell membrane dynamicsprotein synthesis inhibition

    Frequently Asked Questions

    The study shows that junctions form from preexisting membrane-associated components.

    Cycloheximide blocks protein synthesis but does not prevent junction assembly.

    Dinitrophenol inhibits metabolism but junctions still assemble rapidly.

    New junctions are six times longer than apical junctions within five minutes.

    Electron microscopy is used to measure junction length at five-minute intervals.

    Related Experiment Videos

    Main Methods:

    The study uses excised rat prostate tissue incubated at 37 degrees Celsius. Researchers observe tight junction assembly using electron microscopy techniques. They measure junction length changes over time intervals. Protein synthesis is blocked using cycloheximide as a treatment. Metabolic activity is inhibited with dinitrophenol as an alternative. Tissue is fixed at five-minute intervals for microscopic analysis. The lateral plasma membranes of epithelial cells are examined. The presence of new junctions is compared to baseline levels.

    Main Results:

    New tight junctions assemble within five minutes of incubation. These junctions reach six times the length of apical junctions. Assembly occurs even when protein synthesis is inhibited. Metabolic uncouplers do not prevent junction proliferation. The findings suggest preexisting components reorganize rapidly. No new protein synthesis is required for junction assembly. The results indicate a pool of membrane-associated materials is available. The data show that junction proliferation is independent of biosynthesis.

    Conclusions:

    The authors propose that tight junction assembly involves reorganization of existing components. They suggest that junction proliferation occurs without new protein synthesis. The findings imply that junctions can rapidly respond to environmental changes. The study shows that metabolic inhibitors do not block junction assembly. The results indicate that junctions may emerge from preexisting pools. The authors suggest that stress conditions trigger junction proliferation. They propose that fascia occludens may reflect this rapid assembly process. The conclusions highlight a novel mechanism for junction plasticity.

    The authors propose that stress may trigger junction proliferation from preexisting components.