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Related Experiment Videos

Nucleosomes arrangement in chromatin

C Marion, B Roux

    Nucleic Acids Research
    |November 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Chromatin structure changes significantly beyond six nucleosomes, transitioning from negative to positive birefringence. This reveals a helical hexanucleosome structure, impacting DNA organization.

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    Area of Science:

    • Molecular Biology
    • Biophysics
    • Chromatin Structure Analysis

    Background:

    • Nucleosomes are the fundamental repeating units of chromatin, composed of DNA and histone proteins.
    • Understanding nucleosome arrangement is crucial for comprehending DNA packaging and gene regulation.

    Purpose of the Study:

    • To investigate the spatial arrangement and electro-optical properties of nucleosome chains in rat liver chromatin.
    • To determine the structural transition point in chromatin subunits using electric birefringence.

    Main Methods:

    • Electric birefringence technique applied to study chromatin subunits of varying lengths (up to 9 nucleosomes).
    • Analysis of relaxation times and orientation mechanisms to model nucleosome and subunit structures.

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    Main Results:

    • Nucleosomes modeled as oblate ellipsoids (140x140x70 A) with DNA superhelical axis parallel to the shorter axis.
    • A sharp transition in electro-optical properties observed for subunits with more than 6 nucleosomes.
    • Hexanucleosomes exhibit no birefringence, possessing a spherical helical structure (approx. 250 A diameter) without a preferred orientation axis.

    Conclusions:

    • Chromatin subunits undergo a significant structural and electro-optical transition beyond the hexanucleosome stage.
    • The hexanucleosome represents a key structural unit with a symmetrical helical arrangement.
    • Chromatin organization appears to be a periodic array of these hexanucleosome structures.