Serological identification of the new cold agglutinin specificity anti-Gd

The specificity anti-Gd of human cold autoagglutinins is characterized using untreated and enzyme-treated human red blood cells. Gd determinants of human RBC are resistant to proteases, but are inactivated by neuraminidase (RDE). In contrast, I/i determinants are not inactivated by proteases or RDE, while Pr1-3 determinants are inactivated by proteases and RDE, and Pra determinants are resistant to RDE, but are inactivated by proteases.