Related Experiment Video
Updated: Aug 12, 2026

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein
Published on: January 28, 2013
[Synthesis of retinyl phosphate, its physico-chemical properties and biological activity]
Abstract:
Retinyl phosphate (RP) has been synthesized from retinol (R), using phosphodimorpholine chloride (PDMC) or POCl3. A method for isolating and purifying RP from the reaction mixture has been developed. The RP yield varies from 3 to 6% depending on the quantities of R and POCl3. The RP yield can be increased by 10--15% as a result of RP separation and rerun of the reaction. The resultant RP has an absorption maximum at 325 nm, is described by the formula C20H27PO4(NH4)2 and distributed between equal volumes of diethyl ester and 20% aqueous methanol at a ratio of 1:1. RP is alkaline-resistant and rapidly transforms into anhydroretinol (AR) in the acid medium. Eighteen hours after RP administration into the caudal vein or parenterally retinyl palmitate and R are accumulated in the liver of A-avitaminous rats.
More Related Videos
05:03Quantitative Analysis of Dietary Vitamin A Metabolites in Murine Ocular and Non-Ocular Tissues Using High-Performance Liquid Chromatography
Published on: December 27, 2024
08:18Methodology for Studying Interactions of Vitamin A Membrane Receptors and Opsin Protein with their Ligands in Generating the Retinylidene Protein
Published on: October 4, 2024
Related Concept Videos
The Retina
Phosphoinositides and PIPs
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Overview of Fatty Acid Metabolism
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Synthesis of Phosphatidylcholine in the ER Membrane
The major components of all eukaryotic cell...
Photoreceptors and Visual Pathways
Phase II Reactions: Methylation Reactions
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...