[Reaction between prolactin and serum proteins. II. Prolactin-binding gamma globulin of normal animal serum]

The authors identified prolactin-binding proteins in the blood of goats and rats. Proteins were isolated by affine chromatography. It was shown by gel filtration on Sephadex G-200 and Sepharose-6B and also by disc electrophoresis in polyacrylamide gel and zonal electrophoresis in agarose that these proteins were referred to gamma-globulins. In zonal electrophoresis prolactin complex with the binding protein dissociated. Immunoelectrophoresis showed that prolactin-binding proteins were referred to IgA class.