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Equine marker genes: Polymorphism for soluble erythrocyte malic enzyme
Animal Blood Groups and Biochemical Genetics
|January 1, 1981
Summary
Equine erythrocyte malic enzyme (ME1S) exhibits polymorphism detectable via starch gel electrophoresis. Frequencies varied between Standardbred and Thoroughbred horse breeds, with no variation found in soluble malate dehydrogenase.
Area of Science:
- Biochemistry
- Genetics
- Veterinary Science
Background:
- Malic enzyme is crucial in cellular metabolism.
- Understanding genetic variation in equine enzymes aids breed characterization and genetic studies.
Purpose of the Study:
- To investigate the genetic polymorphism of equine erythrocyte malic enzyme.
- To determine the frequency of malic enzyme variants in Standardbred and Thoroughbred horses.
- To assess for genetic variation in soluble malate dehydrogenase.
Main Methods:
- Starch gel electrophoresis was employed to detect enzyme polymorphism.
- Blood samples from Standardbred and Thoroughbred horses were analyzed.
Main Results:
- Polymorphism in equine erythrocyte malic enzyme (ME1S) was successfully detected.
- The frequency of ME1S was 0.06 in 667 Standardbred horses and 0.09 in 85 Thoroughbred horses.
- No genetically determined electrophoretic variation was observed in soluble malate dehydrogenase.
Conclusions:
- Equine erythrocyte malic enzyme exhibits detectable genetic polymorphism.
- Breed-specific frequencies of ME1S exist between Standardbred and Thoroughbred populations.
- Soluble malate dehydrogenase appears to be monomorphic in the studied equine populations.