Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sensitive radioimmunoassay for vancomycin

K L Fong, D H Ho, L Bogerd

    Antimicrobial Agents and Chemotherapy
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A mechanistic look at the effects of adversity early in life on cardiovascular disease risk during adulthood.

    Acta physiologica (Oxford, England)·2013
    Same author

    Epigenetics and transgenerational transfer: a physiological perspective.

    The Journal of experimental biology·2009
    Same author

    Oral uracil and Ftorafur plus leucovorin: pharmacokinetics and toxicity in patients with metastatic cancer.

    Cancer chemotherapy and pharmacology·2000
    Same author

    A binding site for the kringle II domain of prothrombin in the apple 1 domain of factor XI.

    The Journal of biological chemistry·2000
    Same author

    The role of high molecular weight kininogen and prothrombin as cofactors in the binding of factor XI A3 domain to the platelet surface.

    The Journal of biological chemistry·2000
    Same author

    Factor XI binding to activated platelets is mediated by residues R(250), K(255), F(260), and Q(263) within the apple 3 domain.

    Biochemistry·2000
    Same journal

    Isavuconazole for invasive mold disease in patients with hematological malignancies: a multicenter real-world study from China on efficacy, safety, and competing risks.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    An Achilles' heel for methicillin-resistant <i>Staphylococcus aureus</i>? Re-evaluating β-lactam susceptibility of MRSA.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    Essential role of glycogen synthase kinase 3 in regulating growth, drug response, and infectivity in <i>Leishmania infantum</i>.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    <i>In vitro</i> antibacterial activity of gepotidacin in combination with other antimicrobial agents against <i>Neisseria gonorrhoeae</i> isolates.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    Development of domain-specific probes of <i>Plasmodium falciparum</i> heat shock protein 70-1.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    Addressing therapeutic options for KPC-3-producing ST307-<i>Klebsiella pneumoniae</i>: insights from <i>in vitro</i> evolution and mutant prevention strategies.

    Antimicrobial agents and chemotherapy·2026
    See all related articles

    A new radioimmunoassay for vancomycin offers significantly improved sensitivity for detecting drug levels in patient samples. This method accurately measures vancomycin concentrations, even after oral administration, outperforming traditional bioassays.

    Area of Science:

    • Clinical Chemistry
    • Immunology
    • Pharmacology

    Background:

    • Accurate monitoring of vancomycin levels is crucial for patient safety and treatment efficacy.
    • Existing bioassays for vancomycin have limitations in sensitivity and detection after oral administration.

    Purpose of the Study:

    • To develop and validate a highly sensitive radioimmunoassay (RIA) for quantifying vancomycin in biological samples.
    • To compare the performance of the developed RIA with existing bioassays.

    Main Methods:

    • Development of a rabbit antiserum against vancomycin-bovine serum albumin conjugates.
    • Labeling of vancomycin with either tritium (3H) or iodine-125 (125I) as tracers.
    • Validation of the RIA method using serum and urine samples from patients.

    Related Experiment Videos

    Main Results:

    • The developed RIA demonstrated high sensitivity (0.04-4 ng/ml), a 200- to 20,000-fold improvement over bioassays.
    • Vancomycin levels in serum and urine were measurable without sample processing.
    • RIA results correlated well with bioassay for intravenously administered vancomycin.
    • RIA uniquely detected vancomycin after oral administration, where bioassays failed.
    • The antiserum showed no significant cross-reactivity with other antibiotics or chemotherapeutic agents.

    Conclusions:

    • The developed radioimmunoassay provides a sensitive, reproducible, and accurate method for vancomycin quantification.
    • This RIA is particularly valuable for monitoring vancomycin therapy, especially after oral administration.
    • The method's specificity ensures reliable results in the presence of other medications.