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Updated: Aug 11, 2026

Double Whole Mount in situ Hybridization of Early Chick Embryos
Published on: October 27, 2008
[Development of double loach (Misgurnus fossilis) embryos in experimental polyembryony]
Abstract:
After centrifugation of the loach (Misgurnus fossilis) roe, performed immediately after insemination, embryos with double anterior and common posterior body ends are forming, which is considered as a result of noncompleted experimental polyembryony. Twin embryos have been histologically studied at stages 33, 37 and 40 after A.A. Kostomarova (1975). In the experimental embryos normal axiality, as well as the anlage of the germ axial complex are preserved. Nevertheless, in most cases general disorders in their internal structure are observed, such as: underdevelopment, or lack of the chords, certain divergence in the neural tube morphogenesis and in the mesoderm differentiation, unpaired anlage of the organs of sense. There are cases when one or several neural tubes are layed down without any effect of the chorda. The data obtained support B.P. Tokin's idea (1977) on a possible peculiar realization of this process as compared to the normal embryogenesis.

