Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Type IIA hyperlipoproteinemic sera decrease the synthesis of hyaluronic acid by cultured human aortic smooth muscle

H Järveläinen, T Rönnemaa, M Tammi

    Atherosclerosis
    |April 1, 1981
    PubMed
    Summary

    Type IIA hyperlipoproteinemia serum alters glycosaminoglycan synthesis in human aortic cells, increasing the sulphated GAGs to hyaluronic acid ratio, a potential factor in atherosclerosis risk.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Empagliflozin attenuates hypoxia-induced heart failure of zebrafish embryos via influencing MMP13 expression.

    Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2024
    Same author

    Dietary Saturated Fat and Bone Health in Young Adults: The Young Finns Cohort.

    Calcified tissue international·2022
    Same author

    Childhood Oral Infections Associate with Adulthood Metabolic Syndrome: A Longitudinal Cohort Study.

    Journal of dental research·2020
    Same author

    Extracellular matrix-cell interactions: Focus on therapeutic applications.

    Cellular signalling·2019
    Same author

    Geographic origin as a determinant of left ventricular mass and diastolic function - the Cardiovascular Risk in Young Finns Study.

    Scandinavian journal of public health·2018
    Same author

    Education as a moderator of genetic risk for higher body mass index: prospective cohort study from childhood to adulthood.

    International journal of obesity (2005)·2017

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Cardiovascular Science

    Background:

    • Type IIA hyperlipoproteinemia is linked to increased atherosclerosis risk.
    • Arterial smooth muscle cells (SMC) synthesize glycosaminoglycans (GAGs), including sulphated GAGs (S-GAGs) and hyaluronic acid (HA).
    • The ratio of S-GAGs to HA in atherosclerotic lesions is altered.

    Purpose of the Study:

    • To investigate the effect of sera from type IIA hyperlipoproteinemic patients on GAG synthesis by human aortic SMC.
    • To determine if altered GAG synthesis contributes to the association between type IIA hyperlipoproteinemia and atherosclerosis.

    Main Methods:

    • Cultured human aortic SMC were incubated with sera from type IIA hyperlipoproteinemic patients and matched controls.
    • Synthesis of S-GAGs and HA was measured.

    Related Experiment Videos

  • Cell proliferation and DNA content were assessed.

    • Main Results:

    • Type IIA sera significantly decreased HA synthesis by 21% (P < 0.001) but did not affect S-GAGs synthesis.
    • The ratio of S-GAGs to HA was significantly higher with type IIA sera compared to control sera.
    • No significant differences were observed in cell number, DNA content, or thymidine incorporation.

    Conclusions:

    • Sera from type IIA hyperlipoproteinemic patients alter the balance of GAG synthesis in human aortic SMC.
    • This disturbance, characterized by reduced HA and a higher S-GAGs/HA ratio, may contribute to the increased atherosclerosis risk associated with type IIA hyperlipoproteinemia.