[Cytochrome P-450 distribution in endoplasmic reticulum membranes of rat liver]

Using electron-dense labelling in combination with solubilization, the localization of cytochrome P-450 in the microsomal membranes was studied. It was shown that cytochrome P-450 is unevenly distributed in the membrane and has 2-3 reactive SH-groups. The molecules of solubilized cytochrome P-450 contain 4-5 reactive SH-groups and show a tendency to aggregate. A comparative study of cytochrome P-450 distribution in the microsomal ghosts membranes in proteoliposomes was carried out.