[Kinetics of the NADH regenerating system using bacterial formate dehydrogenase]

The main kinetic regularities of the NADH regeneration system functioning was studied. A theoretical interpretation of the dependence of the stationary reaction rate in a two-enzyme system with a common cofactor on the enzyme, cofactor and substrate concentrations and the catalytic parameters of individual enzymatic processes was obtained. A mathematical analysis of the dependences of the stationary rate on the content of each enzyme in the system at different activity ratios of each enzyme and within a broad range of initial cofactor concentrations was carried out. The kinetics of regeneration of native NADH and the NADH immobilized on a water-soluble 4-vinylpyridine and acroleine copolymer in a model two-enzyme formate dehydrogenase--NADH dehydrogenase system were investigated.