[Technic for simultaneous recording of the uptake and secretion of 14C-noradrenaline by rat brain synaptosomes]

A procedure has been suggested for simultaneous recording of 14C-noradrenaline active uptake and induced secretion by rat brain synaptosomes. Active noradrenaline uptake was shown to depend on the presence in the incubation medium of Ca2+. Removal of Ca2+ from the incubation medium considerably reduces the intensity of noradrenaline uptake by the synaptosomes. Unlike the induced secretion in potassium depolarization of synaptosomal membranes, spontaneous release of noradrenaline does not depend on the presence of Ca2+ in the incubation medium.