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Reference values for equine peritoneal fluid

M A Brownlow, D R Hutchins, K G Johnston

    Equine Veterinary Journal
    |April 1, 1981
    PubMed
    Summary
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    This study establishes reference values for equine peritoneal fluid, analyzing cell counts and biochemical profiles in horses without peritoneal alterations. Findings provide crucial diagnostic benchmarks for equine veterinary medicine.

    Area of Science:

    • Veterinary Medicine
    • Equine Physiology
    • Clinical Pathology

    Background:

    • Peritoneal fluid analysis is vital for diagnosing equine abdominal diseases.
    • Establishing normal reference ranges is essential for accurate interpretation of diagnostic tests.
    • Limited data exists on comprehensive peritoneal fluid reference values in healthy horses.

    Purpose of the Study:

    • To determine reference values for equine peritoneal fluid parameters.
    • To establish normal ranges for cell counts and biochemical analytes.
    • To provide a baseline for diagnosing peritoneal effusions in horses.

    Main Methods:

    • Analysis of peritoneal fluid from 20 horses undergoing elective euthanasia.
    • Collection of samples ante mortem or within 1 hour post mortem.

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  • Measurement of specific gravity, total protein, nucleated cell counts, differential cell counts, and biochemical analytes (AST, AP, LDH, TB, TP, BUN, glucose, IP, calcium).

    • Main Results:

    • Mean specific gravity: 1.010; Mean total protein: 7.7 g/L.
    • Mean total nucleated cell count: 4.33 x 10^9/L; predominant cells: polymorphonuclear leucocytes (45.2%) and mononuclear phagocytes (47%).
    • Key biochemical values established, with strong correlations noted between peritoneal fluid and serum levels for TP, BUN, glucose, and IP.

    Conclusions:

    • Established reference values for equine peritoneal fluid parameters.
    • Provides essential data for differentiating normal from abnormal peritoneal fluid in horses.
    • Supports the use of peritoneal fluid analysis as a diagnostic tool in equine practice.