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Isolation of Brucella canis

M H Larsson, E O da Costa

International Journal of Zoonoses

|December 1, 1980

Summary

Brucella canis strains were identified from seropositive dogs, including kennel and stray animals. These isolates were confirmed using morphological, staining, biochemical, and serological methods.

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Area of Science:

Veterinary Microbiology
Canine Infectious Diseases

Background:

Brucella canis is a significant pathogen causing brucellosis in dogs.
Accurate identification of Brucella canis strains is crucial for disease control and public health.

Purpose of the Study:

To isolate and characterize Brucella canis strains from seropositive dogs.
To confirm the identity of these isolates using a combination of microbiological and serological techniques.

Main Methods:

Isolation of bacterial strains from blood samples of seropositive dogs.
Morphological and staining characteristics assessment.
Biochemical profiling of the isolates.
Serological confirmation using hyperimmune serum as antigen.

Main Results:

Three strains of Brucella canis (ES 11/78, 215/79, Pr/79) were successfully isolated.
Isolates exhibited characteristic Brucella morphology and staining properties.
Biochemical tests and serological analysis confirmed the identity of the isolates as Brucella canis.

Conclusions:

The study successfully isolated and identified Brucella canis strains from both kennel and stray dogs.
The findings underscore the importance of diagnostic methods for Brucella canis detection in canine populations.
This characterization aids in understanding the epidemiology and management of canine brucellosis.

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