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Comparative study between microscopic agglutination and complement fixation tests in experimental canine
International Journal of Zoonoses
|December 1, 1980
Summary
This study tracked antibody responses in dogs experimentally infected with Leptospira serovars icterohaemorrhagiae and canicola. Agglutinating antibodies persisted longer than complement-fixing antibodies following infection.
Area of Science:
- Veterinary Immunology
- Bacteriology
- Infectious Diseases
Background:
- Leptospirosis is a significant zoonotic disease caused by Leptospira bacteria.
- Understanding canine antibody responses is crucial for diagnosing and managing leptospirosis.
- Specific serovars, such as icterohaemorrhagiae and canicola, are prevalent in dog populations.
Purpose of the Study:
- To evaluate and compare the antibody response in dogs experimentally inoculated with Leptospira serovars icterohaemorrhagiae and canicola.
- To determine the temporal dynamics of antibody production using different serological tests.
Main Methods:
- Experimental inoculation of 30 dogs (15 per serovar) with pathogenic Leptospira.
- Monitoring antibody response during the acute phase of infection.
- Utilizing microscopic agglutination tests (MAT) and complement fixation tests (CFT).
Main Results:
- Complement-fixing antibodies were detected by day 4 post-infection in both groups.
- Agglutinins (MAT antibodies) appeared in circulation by day 7 post-infection when using homologous antigens.
- Complement-fixing antibodies became undetectable by day 56-63 post-infection, while significant titers of agglutinins persisted.
Conclusions:
- Antibody profiles differ between CFT and MAT in experimentally infected dogs.
- Agglutinins demonstrate a more prolonged presence in circulation compared to complement-fixing antibodies following Leptospira infection.
- These findings have implications for serological diagnosis and understanding the duration of antibody-mediated immunity in canine leptospirosis.