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Related Experiment Videos

Comparative study between microscopic agglutination and complement fixation tests in experimental canine

M K Hagiwara, C A Santa Rosa, A A Pinto

    International Journal of Zoonoses
    |December 1, 1980
    PubMed
    Summary

    This study tracked antibody responses in dogs experimentally infected with Leptospira serovars icterohaemorrhagiae and canicola. Agglutinating antibodies persisted longer than complement-fixing antibodies following infection.

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    Area of Science:

    • Veterinary Immunology
    • Bacteriology
    • Infectious Diseases

    Background:

    • Leptospirosis is a significant zoonotic disease caused by Leptospira bacteria.
    • Understanding canine antibody responses is crucial for diagnosing and managing leptospirosis.
    • Specific serovars, such as icterohaemorrhagiae and canicola, are prevalent in dog populations.

    Purpose of the Study:

    • To evaluate and compare the antibody response in dogs experimentally inoculated with Leptospira serovars icterohaemorrhagiae and canicola.
    • To determine the temporal dynamics of antibody production using different serological tests.

    Main Methods:

    • Experimental inoculation of 30 dogs (15 per serovar) with pathogenic Leptospira.
    • Monitoring antibody response during the acute phase of infection.

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  • Utilizing microscopic agglutination tests (MAT) and complement fixation tests (CFT).

    • Main Results:

    • Complement-fixing antibodies were detected by day 4 post-infection in both groups.
    • Agglutinins (MAT antibodies) appeared in circulation by day 7 post-infection when using homologous antigens.
    • Complement-fixing antibodies became undetectable by day 56-63 post-infection, while significant titers of agglutinins persisted.

    Conclusions:

    • Antibody profiles differ between CFT and MAT in experimentally infected dogs.
    • Agglutinins demonstrate a more prolonged presence in circulation compared to complement-fixing antibodies following Leptospira infection.
    • These findings have implications for serological diagnosis and understanding the duration of antibody-mediated immunity in canine leptospirosis.