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Preparation and properties of antisera to Mph-1, the mouse macrophage alloantigen
Mph-1 is an alloantigen demonstrable on mouse peritoneal macrophages. Two lines, B10M.I and C.I, have been bred for use with strains I/StDa or BALB/c to produce appropriate alloantisera. Optimal immunization procedures for production of anti-Mph-1.2 (the common antigen) are described. Antisera against Mph-1.1 have so far been obtained only erratically. Antisera show complement dependent cytotoxicity against normal peritoneal macrophages and cultured blood monocytes but have no obvious effect on peritoneal lymphocytes or cultured blood neutrophils. Absorption studies show that cell for cell normal peritoneum has about fifteen times more antigen than lymph node, spleen, liver or bone marrow and at least eighty times more than thymus. We were unable to use the antiserum to cause passive enhancement of skin grafts in mice.
Mph-1 is an alloantigen demonstrable on mouse peritoneal macrophages. Two lines, B10M.I and C.I, have been bred for use with strains I/StDa or BALB/c to produce appropriate alloantisera. Optimal immunization procedures for production of anti-Mph-1.2 (the common antigen) are described. Antisera against Mph-1.1 have so far been obtained only erratically. Antisera show complement dependent cytotoxicity against normal peritoneal macrophages and cultured blood monocytes but have no obvious effect on peritoneal lymphocytes or cultured blood neutrophils. Absorption studies show that cell for cell normal peritoneum has about fifteen times more antigen than lymph node, spleen, liver or bone marrow and at least eighty times more than thymus. We were unable to use the antiserum to cause passive enhancement of skin grafts in mice.