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Plasma creatine kinase isoenzymes in the Bar harbor dystrophic mouse
Abstract:
Increased activity of the MM isoenzyme of creatine kinase (CK) was found in plasma from Bar Harbor dystrophic mice of the 129/ReJ dy/dy strain when compared to the findings in non-dystrophic controls. Total plasma CK activity was only slightly increased in dystrophic animals but plasma from both normal and dystrophic mice showed large amounts of the BB isoenzyme of creatine kinase. This isoenzyme was also found to be present in mouse platelets. It is concluded that BB isoenzyme from platelets could have obscured the contribution of muscle CK to the total plasma CK activity and the CK isoenzyme quantitation is needed to evaluate muscle disorders in rodents.
Insights
Increased creatine kinase MM isoenzyme activity was detected in dystrophic mice plasma. Platelet BB isoenzyme masked muscle creatine kinase contributions, necessitating isoenzyme analysis for rodent muscle disorder evaluation.
Area of Science:
- Biochemistry
- Genetics
- Animal Models
Background:
- Muscular dystrophies are genetic disorders characterized by progressive muscle degeneration.
- Creatine kinase (CK) is an enzyme crucial for energy metabolism in muscle tissue.
- CK isoenzymes, including MM and BB, can indicate tissue-specific damage.
Purpose of the Study:
- To investigate creatine kinase (CK) isoenzyme activity in Bar Harbor dystrophic mice.
- To determine the contribution of different CK isoenzymes to plasma CK levels in muscular dystrophy.
- To assess the utility of CK isoenzyme analysis in diagnosing muscle disorders in rodent models.
Main Methods:
- Plasma samples were collected from dystrophic (129/ReJ dy/dy) and non-dystrophic control mice.
- Total plasma CK activity was measured.
- Plasma CK isoenzyme levels (MM and BB) were quantified.
- CK isoenzyme presence in mouse platelets was examined.
Main Results:
- Elevated MM isoenzyme activity of creatine kinase (CK) was observed in dystrophic mice plasma compared to controls.
- Total plasma CK activity showed only a slight increase in dystrophic animals.
- Both normal and dystrophic mouse plasma exhibited significant levels of the BB isoenzyme of CK.
- The BB isoenzyme was also detected in mouse platelets.
Conclusions:
- Platelet-derived BB isoenzyme can obscure the detection of muscle-derived MM isoenzyme in plasma.
- CK isoenzyme quantitation is essential for accurate evaluation of muscle disorders in rodent models.
- This finding highlights the importance of considering non-muscle sources of CK in diagnostic assessments.