Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium component to action potentials in rat pars intermedia cells

W W Douglas, P S Taraskevich

    The Journal of Physiology
    |December 1, 1980
    PubMed
    Summary

    Rat pars intermedia cells exhibit sodium-dependent action potentials, with a secondary calcium-dependent component. This calcium component is crucial for regulating cellular secretion.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Polymyositis-dermatomyositis-associated interstitial lung disease.

    American journal of respiratory and critical care medicine·2001
    Same author

    High short-term mortality following lung biopsy for usual interstitial pneumonia.

    The European respiratory journal·2001
    Same author

    Idiopathic pulmonary fibrosis: Impact of oxygen and colchicine, prednisone, or no therapy on survival.

    American journal of respiratory and critical care medicine·2000
    Same author

    Potassium-induced secretion of melanocyte-stimulating hormone from the melanotrophs of the neurointermediate lobe of the lizard Anolis carolinensis.

    General and comparative endocrinology·1999
    Same author

    Radiation-induced pneumonitis in the "nonirradiated" lung.

    Mayo Clinic proceedings·1999
    Same author

    Colchicine versus prednisone in the treatment of idiopathic pulmonary fibrosis. A randomized prospective study. Members of the Lung Study Group.

    American journal of respiratory and critical care medicine·1998

    Area of Science:

    • Neuroscience
    • Cell Physiology

    Background:

    • Pars intermedia cells in rats play a role in regulating physiological processes.
    • Action potentials are fundamental electrical events in excitable cells.

    Purpose of the Study:

    • To investigate the ionic basis of action potentials in rat pars intermedia cells.
    • To elucidate the contribution of different ions to the electrical activity of these cells.

    Main Methods:

    • Intracellular recording techniques were employed in rat pars intermedia cells.
    • Pharmacological agents like tetrodotoxin (TTX) and tetraethylammonium (TEA) were used to block specific ion channels.
    • Solutions with varying ionic compositions (Na-free, Ca-free) were utilized to assess ion dependency.

    Main Results:

    • Action potentials were primarily dependent on sodium (Na) ions, as evidenced by TTX blockade.
    • TTX-insensitive regenerative potentials were observed, dependent on calcium (Ca) ions.
    • Calcium action potentials were suppressed by Ni, Co, and Mn ions.
    • Strontium (Sr) and barium (Ba) ions could substitute for Ca, prolonging action potential duration.

    Conclusions:

    • Rat pars intermedia cells possess both Na-dependent and Ca-dependent components in their action potentials.
    • The identified Ca component suggests a role for these action potentials in regulating cellular secretion.

    Related Experiment Videos