Partial purification procedure for human urinary erythropoietin by preparative isotachophoresis

Human urinary erythropoietic-active crude protein was isotachophoretic fractionated in a LKB Uniphor apparatus equipped with a plastic column using polyacrylamide as a stabilizing medium, phosphat ions as leading and glycinat ions as terminating electrolytes and Ampholine carrier ampholytes (pH: 5--7) as spacers. The exhypoxic polycythemic mouse method to assay erythropoietic activity showed that the majority of the hormon was sharply resolved. Under these conditions preparative isotachophoresis demonstrated a higher purification factor compared to other biochemical preparation-methods.