Radioimmunoassay for detection of anti-oligodendrocyte antibodies

A solid phase radioimmunoassay (RIA) for detection and quantitation of rabbit anti-oligodendrocyte antibody has been developed using bovine oligodendroglia preparation. The assay is simple, rapid, reproducible and economical. It is approximately 150 x as sensitive as immunofluorescence. Specificity has been established by using different bovine tissue antigens as substrate and absorption studies. This assay represents a potentially powerful tool for the detection and quantitation of anti-oligodendroglial antibodies and oligodendroglial antigens in serum and CSF of man and experimental animals.