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A new tick cell line derived from Boophilus microplus

P J Holman, N C Ronald

    Research in Veterinary Science
    |November 1, 1980
    PubMed
    Summary

    A new tick cell line, Boophilus microplus IX, was established from embryonic cells. This epithelial-like cell line, maintained since 1979, has a male diploid chromosome number of 21.

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    Area of Science:

    • Veterinary Entomology
    • Cell Biology
    • Acarology

    Background:

    • The cattle tick, Boophilus microplus, poses significant economic challenges in livestock production.
    • Continuous cell lines are crucial for research on tick biology and control strategies.

    Purpose of the Study:

    • To report the development and characterization of a new tick cell line derived from Boophilus microplus.
    • To provide a resource for future studies on tick physiology and pathogen interactions.

    Main Methods:

    • Development from primary embryonic cell cultures of Boophilus microplus eggs (6-9 days old).
    • Maintenance in a specialized medium (MEM:L-15, 20% FBS, 10% TPT, 0.1% BSA) at 32°C.
    • Characterization through chromosomal analysis (diploid number 2n=21) and morphological assessment (epithelial-like).

    Main Results:

    • Successful establishment of the Boophilus microplus IX continuous cell line since March 1979.
    • Cells exhibit epithelial-like morphology and a stable male diploid chromosome number (2n=21).
    • The cell line has been maintained for over 40 years, reaching the 26th passage.

    Conclusions:

    • The Boophilus microplus IX cell line is a viable and stable resource for tick research.
    • This cell line can facilitate studies on tick genetics, physiology, and the development of novel control methods.
    • The established cell line offers a platform for investigating tick-borne diseases and improving animal health.

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