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Updated: Aug 14, 2026

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
Published on: August 28, 2018
Effect of heterospecific DNA on the development of Rana nigromaculata
Abstract:
Both the DNA of a balancer-bearing species (Cynops orientalis) and that of a non-balancer-bearing species (Rana nigromaculata), when injected into the fertilized eggs of R. nigromaculata, cause some tadpoles derived from the injected eggs to bear a single balancer-like process on the left operculum. Based upon these results, it may be so concluded that the occurrence of the balancer-like process in the tadpole is neither due to genetic transformation, nor due to balancer induction, but rather due to a non-specific developmental effect of DNA.
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