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Published on: November 27, 2016
Bile acid composition affecting cholesterol dissolution rate: a use of multiple regression analysis
The Tohoku Journal of Experimental Medicine
|April 1, 1981
Summary
Cholesterol dissolution in bile is significantly influenced by deoxycholic and chenodeoxycholic acid concentrations. Bile acid composition impacts cholesterol solubilization, suggesting chenodeoxycholic acid may be more effective for cholesterol gallstones.
Area of Science:
- Hepatology
- Gastroenterology
- Biliary Physiology
Background:
- Cholesterol gallstones are a common condition.
- Bile composition, particularly lipid and bile acid concentrations, plays a crucial role in cholesterol solubility and gallstone formation.
Purpose of the Study:
- To quantify the dissolution rate of cholesterol in human gallbladder bile.
- To investigate the influence of specific bile acids on cholesterol solubilization and compare their potential cholelitholytic effects.
Main Methods:
- Utilized a rotating-disk method with 14C-labeled cholesterol to measure dissolution rates.
- Employed multiple regression analysis to correlate dissolution rates with biliary lipid concentrations.
- Assessed cholesterol solubility in gallbladder bile supplemented with various conjugated bile acids.
Main Results:
- Cholesterol dissolution rate is significantly correlated with deoxycholic acid and chenodeoxycholic acid concentrations (multiple correlation 0.905).
- Greater cholesterol solubilization was observed with added sodium deoxycholylglycine and chenodeoxycholylglycine compared to sodium cholylglycine.
- Sodium ursodeoxycholylglycine did not enhance cholesterol solubility.
Conclusions:
- Deoxycholic acid and chenodeoxycholic acid are key determinants of cholesterol dissolution rate in human gallbladder bile.
- Chenodeoxycholic acid demonstrates a potentially greater cholelitholytic effect than ursodeoxycholic acid, based on its influence on cholesterol solubilization.
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