Investigations concerning the action of serveral chemical and biological agents on HBsAg

Native and purified HBsAg preparations were subjected in vitro to the action of cetylpyridinium bromide (Bromocet), hibitan-chlorhexidine (Hibiscrub), chloramine B and propolis extract, at different concentrations and for various time intervals. The effect of these agents on the serological reactivity of HBsAg was tested by electroimmunodiffusion (EID) and radioimmunoassay (RIA). Chloramine B and the propolis extract had a significant inhibitory effect-ascertained by both EID and RIA - on purified HBsAg, but not on the native preparation. The inhibition exerted by Bromocet and Hibiscrub indicated by EID results was not confirmed by RIA.