H2O2 release from filtration leukapheresis-procured leukocytes

Filtration leukapheresis-procured leukocytes (FL-leukocytes), which were collected by the elution of filtration columns with vigorous tapping, released a certain amount of H2O2 even in the absence of any phagocytic stimuli. Furthermore, FL-leukocytes, eluted with either gentle or vigorous tapping, exhibited a marked release of H2O2 during phagocytosis. The myeloperoxidase (MPO) activity of FL-leukocytes was lower than that of leukocytes collected by the dextran sedimentation method (DS-leukocytes). The data suggest that the release of both H2O2 and MPO from FL-leukocytes may be related to adverse transfusion reactions and abnormal post-transfusion kinetics of FL-leukocytes due to their toxic effects on living cells.