[Chromatography of formalin-inactivated tick-borne encephalitis viruses on macroporous glass]

The results of further development of methods for chromatographic concentration and purification of tick-borne encephalitis virus on columns with porous glass using formalin-inactivated virus suspensions are presented. The method of adsorption chromatography on porous glass under optimal conditions concentrates inactivated TBE virus 20-40-fold with simultaneous purification from protein by 93%-95%. Inactivated virus was shown to keep on glass better than infectious virus. Gel filtration chromatography removes 99.2%-99.8% of protein impurities from inactivated TBE virus preparations giving a complete or nearly complete "yield" of virus particles. The sequential use of adsorption chromatography and gel filtration produced concentrated, most highly purified TBE virus preparations containing no more than 2 microgram/ml protein. Chromatographic virus preparations were immunologically active in experiments in laboratory animals.