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Updated: Aug 1, 2026

Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons
Published on: July 13, 2015
Distribution of cholinesterases of rabbit skeletal muscle
Abstract:
Two cholinesterase (ChE) fractions were extracted from homogenates of rabbit skeletal muscle. One of them the sarcoplasmic ChE could be dissolved quantitatively with solution A. Following the extraction with solution B the myofibrillar ChE fraction could be recovered. The ChE-activity of the sarcoplasmic extract was about 38% and the activity of myofibrillar extract was about 50% of the ChE-activity of muscle homogenates. According to our calculations about 10-12% of the total ChE-activity is insoluble. By the aid of PAGE seven zones of ChE-activity could be distinguished in the sarcoplasmic extract. The activity of myofibrillar ChE-fraction was obtained by chromatographic purification of LMM. The LMM1 proved to be inhomogeneous with PAGE, however, only one fraction displayed ChE-activity. Immune-serum produced against myosin did not give reaction with purified sarcoplasmic ChE, only with the myofibrillar fraction. Our results suggest that the heterogeneous distribution of muscle ChE-es in different molecular forms is not an artifact.
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