Effects of intraocular irrigating solutions on the electroretinographic b-wave

We studied the effects of physiologic saline, lactated Ringer's solution, and BSS (balanced salt solution) on the electroretinogram of the rabbit in vitro. The b-wave amplitudes decreased when the retina was incubated in lactated Ringer's solution or BSS. These amplitudes recovered to 60% to 70% of the original level when the retina was re-immersed in the control medium. With physiologic saline, the b-wave amplitude recovered only to about 30% after 20 minutes of incubation, and recovery was nil after 60 minutes of incubation time. Extended contact with physiologic saline produced permanent damage to the retina. Bicarbonate ion and glucose were shown to be essential for maintaining the ERG amplitude.