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[In vitro S. mansoni miracidia transformation (author's transl)]
Annales De Parasitologie Humaine Et Comparee
|January 1, 1981
Abstract:
Miracidia de S. mansoni hatched and collected aseptically are able to survive in vitro in suitable media. Development of the miracidia is observed in the same manner that occurs in vivo. They shed their ciliated epidermal cell and miracidium-sporocyst transformation occurs. 30% of the miracidia explanted are transformed in primary sporocystes into 48 h. and are still alive a week later.
Insights
Schistosoma mansoni miracidia can survive and develop in laboratory conditions, mirroring their natural growth. A significant portion transforms into the next larval stage within two days.
Area of Science:
- Parasitology
- Developmental Biology
- Infectious Diseases
Context:
- Schistosoma mansoni is a parasitic flatworm responsible for schistosomiasis.
- Understanding the early life stages of S. mansoni is crucial for disease control.
- In vitro models are valuable for studying parasite development and drug efficacy.
Purpose:
- To investigate the in vitro survival and developmental capacity of Schistosoma mansoni miracidia.
- To observe the transformation process from miracidium to primary sporocyst in a controlled environment.
- To establish a reliable in vitro system for studying early-stage schistosome development.
Summary:
- Aseptically collected Schistosoma mansoni miracidia demonstrate viability and developmental potential in suitable in vitro media.
- The in vitro development recapitulates key in vivo events, including the shedding of the ciliated epidermis and transformation into primary sporocysts.
- Approximately 30% of explanted miracidia successfully transformed into primary sporocysts within 48 hours, with continued viability observed for up to a week.
Impact:
- Provides a foundation for developing novel in vitro assays for schistosome research.
- Facilitates the study of miracidial transformation and potential targets for intervention.
- Supports the development of new strategies for controlling schistosomiasis transmission.