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Published on: November 15, 2013
The effects of substrate flow-rate on immobilized urease assays
Biochimica Et Biophysica Acta
|June 15, 1981
Abstract:
Urease (urea aminohydrolase, EC 3.5.1.5) has been immobilized on o-alkylated nylon tubes in an enzymatically active form. The measured activity of the enzyme coil has been shown to be dependent on the flow rate of substrate through it. The apparent Km decreased and V increased as flow rates were increased. It is suggested that diffusional control is responsible for these observations. Their implications in flow-through immobilized enzyme analyses are discussed.
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01:19Enzyme Kinetics
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
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Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...