[Microgranulated form of cis-cinnamoylchymotrypsin for half-tone enzyme photography]

The microgranules containing covalently bound cis-cinnamoylchymotrypsin were obtained by emulsion polymerization of acrylamide and the cross-linking agent in the presence of a light-sensitive enzyme premodified by acryloylchloride. The kinetics of light reactivation of microgranulated cis-cinnamoylchymotrypsin was studied. It was proposed to develop the latent enzyme image by the reaction of N-acetyl-L-leucine indoxyl ester hydrolysis. The product of this reaction, indoxyl, forms indigo in the presence of oxygen. The intensity of staining was shown to depend on the exposition time. This system can be used for production of half-tone enzyme photomaterials.