Locating spike-initiating or spike-entry zones in a branched molluscan neuron

A theoretical method for locating spike-initiating or spike-entry zones within a single neuron was experimentally verified using the cell RPI in the marine opisthobranch Navanax inermis. Multiple sites of spike-initiation were found to exist in RPI. Furthermore, it was demonstrated that extracellular recording alone was sufficient to map sections of cells' branching patterns and possibly locate multiple spike-initiating zones.