Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fast sodium current in cardiac muscle. A quantitative description

L Ebihara, E A Johnson

    Biophysical Journal
    |November 1, 1980
    PubMed
    Summary

    This study models the fast sodium current in cardiac muscle using Hodgkin-Huxley equations and voltage-clamp data. Results show minimal impact of series resistance on sodium current measurements.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Enamel Etching for Universal Adhesives: Examination of Enamel Etching Protocols for Optimization of Bonding Effectiveness.

    Operative dentistry·2019
    Same author

    Dispersal capacity and seed production in anemochorous plants.

    Oecologia·2017
    Same author

    Moving beyond the cambium necrosis hypothesis of post-fire tree mortality: cavitation and deformation of xylem in forest fires.

    The New phytologist·2012
    Same author

    Optimising the care of patients with cirrhosis and gastrointestinal haemorrhage: a quality improvement study.

    Alimentary pharmacology & therapeutics·2011
    Same author

    Smothering in commercial free-range laying hens: a preliminary investigation.

    The Veterinary record·2011
    Same author

    Mapping with RAD (restriction-site associated DNA) markers to rapidly identify QTL for stem rust resistance in Lolium perenne.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2011

    Area of Science:

    • Biophysics
    • Cardiac Electrophysiology

    Background:

    • The voltage and time-dependence of fast sodium current (INa) in cardiac muscle are crucial for understanding cardiac action potentials.
    • Previous studies have provided experimental data on INa, but a comprehensive model is needed.

    Purpose of the Study:

    • To describe the voltage and time-dependence of the tetrodotoxin-sensitive, fast sodium current in cardiac muscle using the Hodgkin-Huxley formalism.
    • To quantitatively analyze voltage-clamp data from embryonic heart cells to refine the INa model.

    Main Methods:

    • Utilized two-microelectrode voltage-clamp data from Ebihara et al. (1980) on cultured embryonic heart cells at 37°C.
    • Applied the Hodgkin-Huxley model with specific differential equations for gating variables m and h.
    • Simulated membrane current with and without series resistance to assess its effect.

    Main Results:

    • Developed a Hodgkin-Huxley model for INa with defined rate coefficients (alpha m, beta m, alpha h, beta h) dependent on membrane potential.
    • The model's gating kinetics were found to be faster than those reported for squid giant axon.
    • Simulations indicated that series resistance had minimal effects on the shape and magnitude of the inward transient current.

    Conclusions:

    • The developed Hodgkin-Huxley model accurately describes the fast sodium current in cardiac muscle.
    • Experimental data on INa are reliable, even without complete series resistance compensation.
    • The findings contribute to a better understanding of cardiac electrophysiology and sodium channel function.

    Related Experiment Videos