[Computer classification of the fusion profiles of human lymphocyte interphase chromatin]

AO fluorescent microscopy coupled with thermal denaturation of DNP cells as modified by the authors was used to study the structure of lymphocyte interphase chromatin from 164 normal persons. Analysis of the data processed by means of a Sperry Univac Computer 90/30 has demonstrated that in 40% of the cases, the melting profiles of DNP cells from normal persons represent, irrespective of the sex, a complicated but consistently repeated curve with 6 peaks at certain temperatures, i.e. that different test subjects have a pronounced "similarity" in the characteristics discussed. In the remaining cases, there have been recorded diverse but consistent deviations that correlated with the sex of the test subjects. No peak at a temperature of 85 degrees and its appearance at 82 degrees C were the most frequent deviations seen in the male group. There have been obtained altogether 5 subgroups for females and 7 subgroups for males. Thus an attempt has been made to classify for the first time the melting profiles of DNP cells from normal persons with the use of computer.