Determination of steady state generation time distributions from labelled mitosis experimental data

The proper application of detailed deterministic cell kinetic models depends on the way in which cells are assigned their generation times. A method is presented for the determination of population generation time distributions from labelled mitoses experiments. The model assumes that the generation time of each new cell is a function of both the steady-state generation time distribution function of the population, and also the generation time frequency-function of the previous generation of cells. This approach is applied to two different cell types to successfully simulate extended labelled mitoses curves using a population balance model with constant maturation rates.