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Triethylenemelamine (TEM): dominant lethal effects in Fischer 344 rats
Abstract:
Male Fischer 344 rats were administered triethylenemelamine orally at dose levels of 0, 0.5 or 1.0 mg TEM/kg/day, five days per week for four weeks. A separate group of males was administered TEM as a single intraperitoneal injection of 0.3 mg/kg. Following treatment, males were mated with two groups of untreated females for a period of one week each. The uterine contents of untreated females were examined for evidence of a dominant lethal effect as manifested in an increase in the average resorption rate. Significant increases in the resorption rate were seen at 0.5 mg/kg/day for the second breeding period, and at 1.0 mg/kg/day for both breeding periods following oral administration. Significant decreases in the number of implantations, and increases in the average pre-implantation loss and resorption rate were observed following intraperitoneal administration. These effects seen in Fischer 344 rats were comparable to results obtained with other strains following a similar treatment regimen.
Insights
Triethylenemelamine (TEM) exposure in male rats caused reproductive toxicity, indicated by increased resorption rates and reduced implantations. These dominant lethal effects were observed after both oral and intraperitoneal TEM administration.
Area of Science:
- Toxicology
- Reproductive Toxicology
- Genotoxicity
Background:
- Triethylenemelamine (TEM) is a known alkylating agent.
- Assessing the reproductive toxicity of chemical agents is crucial for risk assessment.
Purpose of the Study:
- To evaluate the dominant lethal effects of triethylenemelamine (TEM) in male Fischer 344 rats.
- To determine dose-dependent reproductive toxicity following oral and intraperitoneal TEM administration.
Main Methods:
- Male Fischer 344 rats were administered TEM orally (0, 0.5, 1.0 mg/kg/day) or via single intraperitoneal injection (0.3 mg/kg).
- Treated males were mated with untreated females, and uterine contents were analyzed for implantation and resorption rates.
Main Results:
- Oral TEM administration at 0.5 and 1.0 mg/kg/day significantly increased resorption rates.
- Intraperitoneal TEM administration resulted in decreased implantations and increased pre-implantation loss and resorption rates.
- Observed effects in Fischer 344 rats were consistent with previous studies in other rat strains.
Conclusions:
- Triethylenemelamine (TEM) exhibits significant dominant lethal effects in male rats.
- Both oral and intraperitoneal routes of TEM exposure induce reproductive toxicity.
- Fischer 344 rats are a suitable model for assessing TEM-induced reproductive hazards.