The binding of oestradiol-17beta to human spermatozoa - an electron microscope autoradiographic study

The location of steroid binding sites with specificity for oestradiol-17beta at an ultrastructural level has been examined on human spermatozoa by electron microscope autoradiography. About 75% of the 438 gains produced by 3H-oestradiol-17beta, and counted on electron microscope autoradiographs, were found over the plasma membrane or within 1000 A of it. This study provides evidence suggesting that the plasma membrane is a site of action of oestradiol-17beta in human spermatozoa.