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Free L-carnitine in human seminal plasma
International Journal of Andrology
|June 1, 1981
Summary
Free L(-)-carnitine in seminal fluid primarily originates from the epididymis, not seminal vesicles. This finding helps clarify the source of L(-)-carnitine for assessing male reproductive health.
Area of Science:
- Reproductive Biology
- Andrology
- Biochemistry
Background:
- Seminal plasma L(-)-carnitine is a potential biomarker for epididymal function.
- The precise origin of L(-)-carnitine in human seminal fluid has been debated, with contributions from both the epididymis and seminal vesicles.
Purpose of the Study:
- To investigate the primary source of free L(-)-carnitine in human seminal plasma.
- To determine the contribution of the epididymis versus seminal vesicles to seminal L(-)-carnitine levels.
Main Methods:
- Free L(-)-carnitine was quantified using an enzymatic spectrophotometric method after deproteinization.
- Semen samples were analyzed from men with normal spermiograms, vasectomized men, and men with agenesis of the vas deferens and seminal vesicles.
Main Results:
- Seminal plasma L(-)-carnitine concentrations were significantly higher in men with normal spermiograms (1010 nmoles) compared to vasectomized men (131 nmoles) and men with agenesis (21 nmoles).
- Analysis of split ejaculates and the lack of correlation with fructose suggested a minor contribution from seminal vesicles.
Conclusions:
- Free L(-)-carnitine in seminal fluid is predominantly of epididymal origin.
- Seminal vesicles contribute minimally to the total L(-)-carnitine concentration in human seminal plasma.