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Enzyme-Linked Immunosorbent Assay

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Murine Model of Allergen Induced Asthma

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An immunochemical method to measure atmospheric allergens

M K Agarwal, J W Yunginger, M C Swanson

The Journal of Allergy and Clinical Immunology

|September 1, 1981

Summary

A new immunochemical method effectively captures and quantifies airborne allergens like pollen and fungal spores. This assay correlates well with traditional methods, improving allergen exposure assessment.

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A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

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Published on: June 4, 2017

Area of Science:

Environmental Science
Immunology
Analytical Chemistry

Background:

Particulate aeroallergens, including pollen, fungal spores, and amorphous particles, contribute to allergic disease symptoms.
Accurate measurement of total allergen exposure is crucial for understanding allergic disease correlation.
Existing methods for aeroallergen quantification have limitations in capturing diverse forms and amounts.

Purpose of the Study:

To develop and validate an immunochemical method for assaying airborne particulate aeroallergens.
To quantify allergen content from various airborne particles, including amorphous forms.
To correlate immunochemical measurements with traditional aeroallergen counting methods.

Main Methods:

Airborne particles (≥0.3 µm) were captured on fiberglass filters using a high-volume air sampler.
Allergens were eluted from filters using buffered saline.
Allergen content was analyzed via radioallergosorbent test (RAST) inhibition assays.

Main Results:

An 8-hour descending elution procedure proved optimal for allergen extraction from filters.
The method detected as little as 4 mg of short ragweed (SRW) pollen or Alternaria powder.
Outdoor measurements showed high correlation between atmospheric allergenic activity and traditional pollen/spore counts.

Conclusions:

The developed immunochemical assay is a sensitive and effective tool for quantifying airborne allergens.
This method provides a more comprehensive assessment of allergen exposure compared to traditional counting.
The assay has practical applications in monitoring atmospheric allergenic activity and correlating it with allergic disease prevalence.