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Related Experiment Videos

Inoculation of dengue virus into nude mice

H Hotta, I Murakami, K Miyasaki

    The Journal of General Virology
    |January 1, 1981
    PubMed
    Summary

    Athymic nude mice infected with dengue virus (DV) showed prolonged survival but impaired IgG antibody production compared to heterozygous mice, suggesting thymus-derived lymphocytes influence DV infection course.

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    Area of Science:

    • Immunology
    • Virology
    • Infectious Diseases

    Background:

    • Dengue virus (DV) infection poses a significant global health challenge.
    • Understanding the host immune response is crucial for developing effective treatments.
    • Athymic nude (nu/nu) mice lack functional thymus-derived lymphocytes (T-cells), offering a model to study T-cell roles in viral infections.

    Purpose of the Study:

    • To investigate the impact of T-cell deficiency on the course of dengue virus infection in mice.
    • To compare dengue virus infection dynamics in athymic nude (nu/nu) mice and heterozygous littermates (nu/+).

    Main Methods:

    • Intraperitoneal injection of a mouse-adapted dengue virus strain into nu/nu and nu/+ mice.
    • Monitoring survival rates, viral persistence, antibody production (IgM and IgG), and histopathological changes (mononuclear cell infiltration).
    • Quantification of viral titers in various organs and detection of viral antigen using the fluorescent antibody (FA) technique.

    Main Results:

    • Nu/nu mice exhibited longer survival times but similar mortality ratios compared to nu/+ mice.
    • DV persisted longer in asymptomatic nu/nu mice, which also showed transient IgM but lacked regular IgG antibody production.
    • Nu/+ mice displayed more intense brain inflammation (mononuclear cell infiltration) than nu/nu mice.
    • Higher viral titers were observed in the hearts of nu/nu mice, while brain, muscle, and lymph node viral loads were comparable.

    Conclusions:

    • The availability of thymus-derived lymphocytes (T-cells) significantly affects the course of dengue virus infection in mice.
    • T-cells appear crucial for effective viral clearance and the development of a robust antibody response, including IgG production.
    • The study highlights the complex interplay between the host immune system, specifically T-cells, and dengue virus pathogenesis.

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