Semiautomated assay for indapamide in biological fluids

Area of Science:

• Analytical Chemistry
• Pharmacokinetics
• Biomedical Analysis

Background:

• Indapamide is a diuretic used to treat hypertension.
• Accurate quantification of indapamide in biological matrices is essential for therapeutic drug monitoring and pharmacokinetic studies.
• Existing methods may lack sensitivity or require complex sample preparation.

Purpose of the Study:

• To develop and validate a sensitive fluorescence procedure for determining indapamide concentrations.
• To enable the measurement of indapamide in plasma, whole blood, and urine.
• To support pharmacokinetic investigations of indapamide.

Main Methods:

• Development of a fluorescence-based assay.
• Preextraction of biological samples (plasma, whole blood).
• Continuous-flow analysis for sample processing and detection.

Main Results:

• The assay demonstrates high sensitivity, detecting indapamide at levels as low as 25 ng/ml.
• A linear response was observed for indapamide concentrations ranging from 25 to 200 ng/ml.
• The method was successfully applied to measure indapamide levels in plasma and whole blood from subjects receiving a 2.5 mg dose.

Conclusions:

• A sensitive and reliable fluorescence method for indapamide determination in biological fluids has been established.
• The developed assay is suitable for pharmacokinetic studies and therapeutic drug monitoring of indapamide.
• The procedure allows for the measurement of indapamide in plasma, whole blood, and urine.