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The role of trace elements and phosphates in the synthesis of vascular-permeability factor by Vibrio cholerae

I K Sagar, C N Nagesha, J V Bhat

    Journal of Medical Microbiology
    |August 1, 1981
    PubMed

    Insights

    Vibrio cholerae requires specific trace elements and phosphates for vascular-permeability factor (PF) synthesis. Magnesium is crucial for PF production in both strains, with optimal synthesis dependent on phosphate concentration.

    Area of Science:

    • Microbiology
    • Biochemistry

    Background:

    • Vibrio cholerae is a significant human pathogen.
    • Vascular-permeability factor (PF) plays a role in disease pathogenesis.

    Purpose of the Study:

    • To investigate the trace element and phosphate requirements for PF synthesis by Vibrio cholerae.
    • To determine the optimal conditions for maximizing PF production.

    Main Methods:

    • Culturing Vibrio cholerae strains B1307 and VC12 under varying trace element and phosphate concentrations.
    • Quantifying vascular-permeability factor (PF) synthesis under different media conditions.

    Main Results:

    • Magnesium is essential for PF synthesis in strain VC12.
    • Strain B1307 synthesized small amounts of PF with iron, zinc, and manganese, but maximum synthesis required magnesium.
    • Phosphate concentrations between 0.75-6.00mM controlled PF synthesis in both strains.

    Conclusions:

    • Trace element and phosphate availability significantly impacts Vibrio cholerae's vascular-permeability factor (PF) production.
    • Magnesium and optimal phosphate levels are critical for maximizing PF synthesis in Vibrio cholerae.

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