1H and 13C NMR spectroscopic study of rat organs

Proton signals of "organic" compounds (other than water) were observed in rat organs such as lung, heart, liver, kidney, and spleen and identified as lipids and glucose by 13C NMR study. T1 of the water protons in these organs was measured by Fourier transform to eliminate contribution from the "organic" protons. Observed T1 values for the organs were liver less than spleen approximately equal to lung, in good accordance with values of the free water contents of those organs determined by thermal analysis. From these and other results it is concluded that 1H NMR spectroscopy can be a useful probe for the study of organs in both normal and pathophysiological states of experimental animals and human beings.