Effect of alterations in reaction conditions on vaccinia virus transcription in vitro

Interrupting the template strand of the T7 promoter facilitates translocation of the DNA during initiation, reducing transcript slippage and the release of abortive products.

Journal of molecular biology·2001

Characterization of halted T7 RNA polymerase elongation complexes reveals multiple factors that contribute to stability.

Journal of molecular biology·2001

The specificity loop of T7 RNA polymerase interacts first with the promoter and then with the elongating transcript, suggesting a mechanism for promoter clearance.

Proceedings of the National Academy of Sciences of the United States of America·2000

Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants.

Biochemistry·2000

Characterization of structural features important for T7 RNAP elongation complex stability reveals competing complex conformations and a role for the non-template strand in RNA displacement.

Journal of molecular biology·1999

Studies of contacts between T7 RNA polymerase and its promoter reveal features in common with multisubunit RNA polymerases.

Biochemistry·1999

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Related Experiment Videos

Effect of alterations in reaction conditions on vaccinia virus transcription in vitro

Related Concept Videos

Interrupting the template strand of the T7 promoter facilitates translocation of the DNA during initiation, reducing transcript slippage and the release of abortive products.

Characterization of halted T7 RNA polymerase elongation complexes reveals multiple factors that contribute to stability.

The specificity loop of T7 RNA polymerase interacts first with the promoter and then with the elongating transcript, suggesting a mechanism for promoter clearance.

Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants.

Characterization of structural features important for T7 RNAP elongation complex stability reveals competing complex conformations and a role for the non-template strand in RNA displacement.

Studies of contacts between T7 RNA polymerase and its promoter reveal features in common with multisubunit RNA polymerases.

The role of autocrine motility factor receptor in antiviral immunity.

RSV, influenza, and COVID-19 in hospitalized children: A comparative analysis of the 2025/2026 epidemic season in Poland.

Cytomegalovirus latency and reactivation: Epigenetic control and immune-microenvironment crosstalk.

Circulation and recombination-driven diversity of duck hepatitis B virus in domesticated geese in Guangdong, southern China.

Disparate tetraparvovirus species linked to hepatitis.

The role of cytokines as immunological biomarkers of disease severity during respiratory syncytial virus (RSV) infection in children.

Ultrasound-Triggered Site-Specific Chemistry Redefines Precision Oncology.

EDA complex-enabled, substituent-controlled switch in anthranil reactivity for sequential access to azaaurones.

A multifunctional PKS-NRPS megasynthetase in nematodes produces divergent signaling molecules that control different developmental arrests.

Distinguishing riboflavin auxotrophy from heat-specific adaptation in Escherichia coli.

METTL14-mediated FDX1 m6A methylation inhibited the proliferation of clear cell renal cell carcinoma.

In Situ Tracking of Se Vacancy-Promoted Surface Reconstruction of NiCoSe2 into NiCoOOH during Alkaline Oxygen Evolution.

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Effect of alterations in reaction conditions on vaccinia virus transcription in vitro

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