Stimulation of collagen biosynthesis by platelet homogenate in various cell cultures

Confluent cultures of Balb c/3T3, L-929 and PZ-32 cells were incubated at 37 degrees C for 16 h in the serum-free culture medium supplemented with ascorbic acid, beta-aminopropionitrile and (5(-3) H)proline. An addition of platelet homogenate to the culture medium increased significantly proline incorporation into proteins and synthesis of the hydroxyproline-containing, collagenase-sensitive proteins.