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[In-vitro block of granulocyte chemotaxis with attenuated measles virus]
Abstract:
Live attenuated measles virus preparations, which are in use today in the prophylaxis against measles, were added in vitro to neutrophils from healthy children with negative history both for measles infection and vaccination. After incubation at 37 degree C for 30 min, and washing in Hank's solution, chemotaxis was assayed in Sykes-Moore chambers. Bacterial endotoxin and sera from healthy donors were used to stimulate motility. A significant decrease in chemotaxis was shown by virus-incubated neutrophils as compared to controls. These findings demonstrate that the attenuated measles virus preserves the same ability to inhibit neutrophil chemotaxis than the wild one, during the infection by which an in vivo defective chemotaxis has been previously described. It is suggested that some interference(s) of the virus may occur directly in the cell and/or its membrane receptors.
Insights
Live attenuated measles virus inhibits neutrophil chemotaxis, similar to wild measles virus. This suggests the virus interferes with neutrophil function, potentially impacting immune responses.
Area of Science:
- Immunology
- Virology
Context:
- Measles virus is used for prophylaxis.
- Neutrophil chemotaxis is crucial for immune response.
Purpose:
- To investigate the effect of live attenuated measles virus on neutrophil chemotaxis.
Summary:
- Live attenuated measles virus was added to neutrophils in vitro.
- Neutrophil chemotaxis was significantly decreased after virus incubation.
- The attenuated virus showed similar inhibition of chemotaxis as the wild-type virus.
Impact:
- Suggests measles virus interferes with neutrophil cell function or membrane receptors.
- May explain defective chemotaxis observed during measles infection.
- Highlights potential impact on immune defense mechanisms.